βiii spectrin c terminus (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
βiii spectrin c terminus
βiii Spectrin C Terminus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectrin+c+terminus/pmc05511878-65-8-13?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 18 article reviews
βiii Spectrin C Terminus, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectrin+c+terminus/pmc05511878-65-8-13?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 18 article reviews
βiii spectrin c terminus - by Bioz Stars,
2026-08
93/100 stars
Images
1) Product Images from "βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons"
Article Title: βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.3520-16.2017
Figure Legend Snippet: βIII spectrin is expressed in the hippocampus and cultured hippocampal neurons and localizes to the somatodendritic compartment. A, Quantitative RT-PCR of βI (SPTB), βII (SPTBN1), and βIII (SPTNB2) spectrins in the adult rat cerebellum (CB), hippocampus (HP), and cerebral cortex (CC). Data from two wild-type rats in triplicates are normalized to the expression of tubulin. Error bars indicate SEM. B, Western blots of βII and βIII spectrins in lysates of adult rat cerebellum (CB), hippocampus (HP), and cerebral cortex (CC); α-tubulin (clone DM1α) is used as loading control. C, Immunofluorescence staining of CA1 region of the mouse hippocampus with βIII spectrin antibody. White represents βIII spectrin localization. so, Stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. D, Western blot of βIII spectrin in cell lysates prepared from 7, 14, and 21 DIV hippocampal neurons; tubulin β3 (clone Tuj1) is used as loading control. E, F, Immunofluorescence staining of 17 DIV hippocampal neurons with antibodies to βIII spectrin (magenta) and either the dendritic marker MAP2 (E, green) or the axonal marker SMI312 (F, green). Left panels, Boxed areas are zoomed in right panels as individual channels and merged images. Bars, 20 μm.
Techniques Used: Cell Culture, Quantitative RT-PCR, Expressing, Western Blot, Control, Immunofluorescence, Staining, Marker
Figure Legend Snippet: Subspine distribution of βIII spectrin in brain tissue. A, B, Fluorescence staining with βIII spectrin antibody (magenta) and phalloidin (cyan) of tissue sections from mouse hippocampus (A) and cerebellum (B). C, Immunogold EM of βIII spectrin staining in thin sections of mouse brain. Dendritic spines are pseudocolored in yellow. Insets, Boxed regions are enlarged to show gold particles (5 nm). Synaptic vesicles can be seen on the opposite side of the synapses. Scale bars: A, B, 1 μm; C, 200 nm.
Techniques Used: Fluorescence, Staining
Figure Legend Snippet: βIII spectrin is enriched in the neck and base of dendritic spines. A, Staining of 17 DIV hippocampal neurons with βIII spectrin antibody (magenta) and phalloidin (cyan) shows that βIII spectrin is enriched at dendritic spine bases and necks, but not abundant in spine heads. Top, Boxed region is enlarged in the bottom. Scale bars: Top, 10 μm; Bottom, 2 μm. B, Enlarged views of dendritic spines marked by arrows in A (bottom). C, Percentage of βIII spectrin-positive dendritic protrusions in neurons of different ages (7 DIV: N = 128 dendritic protrusions in 9 cells; 14 DIV: N = 124 protrusions in 6 cells; 17 DIV: N = 100 protrusions in 9 cells; 21 DIV: N = 119 protrusions in 10 cells). D, Staining of 14 DIV hippocampal neurons with phalloidin and antibodies against βIII spectrin and PSD95. Scale bar, 5 μm. E, Enlarged views of dendritic spines marked by arrows in D. F, Expression of GFP-βIII spectrin in 14 DIV hippocampal neurons followed by staining with antibodies to GFP (green), MAP2 (blue), and PSD95 (magenta). Top, Merged image of a dendrite. Boxed area is zoomed below as individual channels and a merged image. Scale bar, 5 μm. G, Quantification of subspine distribution of βIII spectrin in 17 DIV cultured rat hippocampal neurons (208 spines from 9 dendrite segments) and tissue sections from mouse hippocampus (74 spines from 18 dendrite segments) and cerebellum (126 spines from 18 dendrite segments).
Techniques Used: Staining, Expressing, Cell Culture
Figure Legend Snippet: Loss of βIII spectrin functions inhibits formation of dendritic spines. A, Western blotting of βIII spectrin in lysates of 17 DIV neurons transfected with control (shc) or βIII spectrin-targeting shRNAs (sh#1 and sh#2). Tubulin β3 (Tuj1) is used as loading control. B, Fluorescence staining of 17 DIV neurons transfected with control shRNA (shc, left) or βIII spectrin shRNA #2 (sh#2, right) with phalloidin (green) and βIII spectrin antibody (magenta). Scale bar, 10 μm. Top, Boxed areas are enlarged at bottom. C, Average number of dendritic spines per 100 μm of MAP2-labeled dendrites in neurons infected with control (shc) or βIII spectrin (sh#1 and sh#2) shRNAs (shc: N = 651 spines in 9 cells; sh#1: N = 199 spines in 9 cells; sh#2: N = 169 spines in 10 cells). Error bars indicate SD. ***p = 0.00013 (Dunn's Multiple Comparisons Test). **p = 0.0013 (Dunn's Multiple Comparisons Test). D, Fluorescence staining of 17 DIV neurons transfected with control shRNA (shc, left) or βIII spectrin shRNA #2 (sh#2, right) with phalloidin (green), MAP2 (blue), and PSD95 antibody (red). Scale bar, 10 μm. Top, Boxed areas are enlarged at bottom. E, Average number of PSD95 clusters per 100 μm of MAP2-labeled dendrites (shc: N = 1261 clusters in 9 cells; sh#1: N = 199 clusters in 9 cells; sh#2: N = 443 clusters in 10 cells) in neurons transfected with control (shc) or βIII spectrin (sh#1 and sh#2) shRNAs. Error bars indicate SD. ***p = 0.0014 (Dunn's Multiple Comparisons Test). *p = 0.046 (Dunn's Multiple Comparisons Test). F, Average percentage of PSD95 clusters associated with dendritic spines versus dendrite shafts (shc: N = 1155 clusters in 9 cells; sh#1: N = 961 clusters in 8 cells; sh#2: N = 1791 clusters in 10 cells). Error bars indicate SD. ***p = 0.000012 (Dunn's Multiple Comparisons Test). *p = 0.014 (Dunn's Multiple Comparisons Test).
Techniques Used: Western Blot, Transfection, Control, Fluorescence, Staining, shRNA, Labeling, Infection
Figure Legend Snippet: Depletion of βIII spectrin results in formation of aberrant synapses. A, B, Fluorescence staining of 17 DIV neurons transfected with control shRNA (shc) or βIII spectrin shRNA (sh#2) with phalloidin, axonal marker SMI312, and synapsin I antibody. Scale bar, 10 μm. B, High-magnification images showing spiny synapses in a control cell (top) and shaft synapses in a βIII spectrin-depleted cell (bottom). Scale bar, 5 μm. C, Average numbers of synapsin I puncta per 100 μm of axon length (shc: N = 664 puncta in 9 cells; sh#1: N = 631puncta in 10 cells; sh#2: N = 615 puncta in 10 cells). Error bars indicate SD. D, Staining of PSD95 and synapsin I in control (shc) and βIII spectrin-depleted (sh#2) cells. Scale bar, 5 μm. Boxed areas are zoomed at right. E, Quantification of synapsin I puncta associated with “bright” or “weak” PSD95 puncta or not associating with PSD95 puncta (“synapsin I only”) in control and βIII spectrin-depleted cells (shc, N = 694 puncta in 5 cells; sh#2, N = 578 puncta in 5 cells).
Techniques Used: Fluorescence, Staining, Transfection, Control, shRNA, Marker
Figure Legend Snippet: PREM of dendritic spine cytoskeleton in 17 DIV hippocampal neurons. A–C, Nonactin filamentous components in the dendritic spine cytoskeleton. A, Dendritic spine with actin filaments decorated by myosin S1. Cytoskeletal components within the yellow box at the spine base are pseudocolored as the following: purple represents S1-decorated actin filaments; red represents microtubules; green represents undecorated thin fibrils. B, C, Enlarged white boxes (b and c, respectively) from A showing S1-decorated actin filaments (arrowheads) and undecorated thin fibrils (arrows). B, Bottom left arrow indicates a fine network of undecorated thin fibrils. D, Dendritic spine labeled by βIII spectrin immunogold. Gold particles (12 nm, pseudocolored in yellow) label the spine base and neck but are sparse in the spine head. White-framed inset, Color-coded version of the same image showing the dendritic spine in cyan and axon in magenta. Yellow box is enlarged in the yellow-framed inset and color-coded to show gold particles (yellow), actin filaments (purple), and spectrin molecules (green). E, Immunogold PREM with septin 7 antibody. Top, Overview of a dendritic spine. Inset, Color-coded version of the same image showing the dendrite and dendritic spine in cyan and axon in magenta. Middle, Bottom, Enlarged areas outlined by yellow and blue boxes in the top panel. Gold particles (18 nm, pseudocolored in yellow) associate with rough-contoured filaments (pseudocolored in red) at the spine base (bottom) and in a putative presynaptic compartment of the axon (middle). Scale bars, 200 nm.
Techniques Used: Labeling
Figure Legend Snippet: PREM of synapses in control and βIII spectrin knockdown neurons at 17 DIV. A–E, Neurons treated with control shRNA. A, Dendritic spine (yellow) forms a synapse with a presynaptic bouton in the axon (magenta) in nonextracted cells. B, Correlative fluorescence microscopy and PREM of synapses formed on dendritic spines in neurons fluorescently stained with phalloidin (green) and synapsin I (magenta). Fluorescence image is superimposed onto the PREM image in the main panel and shown separately in the inset. C, Synaptophysin immunogold PREM of a dendritic spine (green) making a synapse with a presynaptic bouton (red) labeled with gold particles (pseudocolored in blue). Inset, Overview of the spine. D, Varicosity on a putative axon (magenta) of a control neuron with a collection of vesicles seen through spontaneous perforations of the plasma membrane; it likely corresponds to an orphan bouton. E, Unapposed presynaptic bouton in a control neuron revealed by synaptophysin immunogold PREM. Membranes are perforated due to detergent extraction after glutaraldehyde fixation. F–K, Neurons treated with βIII spectrin shRNA #2. F, A bundle of neurites making extensive lateral contacts in nonextracted cells (individual neurites are shaded in different colors). G–I, Synaptophysin immunogold PREM (gold particles pseudocolored in blue) of a bundle of neurites (shaded in different colors). Boxed regions are zoomed in H (white box) and I (red box). H, Gold particles label an ellipsoid bouton in the putative axon (red-shaded neurite), which makes a shaft synapse with an adjacent putative dendrite. I, Gold-labeled globular varicosity on a putative axon. It may form a shaft synapse with the underlying “dendrite” or correspond to an orphan bouton. J, A putative orphan bouton (magenta) in nonextracted neuron; a collection of vesicles is seen through spontaneous perforations of the plasma membrane. K, An orphan bouton revealed by synaptophysin immunogold PREM (gold particles pseudocolored in blue). Scale bars: A, B, D, F, J, 0.5 μm; G, 1 μm; C, E, H, I, K, 0.2 μm.
Techniques Used: Control, Knockdown, shRNA, Fluorescence, Microscopy, Staining, Labeling, Clinical Proteomics, Membrane, Extraction
Figure Legend Snippet: Knockdown of βIII spectrin increases amplitudes of spontaneous mEPSCs. A, Representative 10 s traces of spontaneous mEPSCs in DIV17 transfected with βIII spectrin shRNA #2 (knockdown) or a control shRNA (control). Whole-cell patch-clamp recordings were obtained under voltage-clamp configuration with a holding potential of −70 mV. Each trace is from a different hippocampal neuron from the two experimental groups. B, The distribution of mESPC amplitudes is shifted toward higher values. *p = 0.034 (Kolmogorov–Smirnov test). The mean event amplitude is significantly higher in βIII spectrin knockdown neurons (19.48 ± 1.00 pA, n = 205 events from 11 cells) compared with controls (16.21 ± 0.56 pA, n = 201 events from 10 cells). **p = 0.004 (unpaired Student's t test). C, The distribution of mEPSC interevent intervals (p = 0.219, Kolmogorov–Smirnov test) and the mean interevent interval is unchanged in βIII spectrin knockdown (0.48 ± 0.039 s) compared with control neurons (0.44 ± 0.047 s; p = 0.497, unpaired Student's t test).
Techniques Used: Knockdown, Transfection, shRNA, Control, Patch Clamp
Figure Legend Snippet: Model of βIII spectrin functions in determination of the dendritic spine shape. Left, βIII spectrin-containing tetramers together with actin filaments form a membrane skeleton in the dendrite and at the base and neck of dendritic spines. This network helps to stabilize the dendritic spine base and constrict the dendritic spine neck. A narrow neck forms a diffusion barrier that controls biochemical and electrical communication through the spine neck and limits a loss of the turning over PSD components from the spine head (green arrow). Right, In the absence of functional βIII spectrin, the constricted neck is not formed, leading to an increased formation of shaft synapses (top) and nonsynaptic boutons (bottom). Uncontrolled communication between the synapse and the parent dendrite in shaft synapses results in increased amplitudes of spontaneous mEPSCs and a partial loss of postsynaptic components from the synapse (green arrow).
Techniques Used: Membrane, Diffusion-based Assay, Functional Assay